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maxiscript t7 in vitro transcription kit  (Thermo Fisher)


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    Thermo Fisher maxiscript t7 in vitro transcription kit
    Maxiscript T7 In Vitro Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maxiscript+t7+in+vitro+transcription+kit/maxiscript+t7+transcription+kit/pmc12112137-260-18-18
    Average 90 stars, based on 1 article reviews
    maxiscript t7 in vitro transcription kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    In Vitro:

    Article Title: Suppression of host plant defense by bacterial small RNAs packaged in outer membrane vesicles.
    Article Snippet: .. The MAXIscript T7 In Vitro Transcription Kit (ThermoFisher, 445 USA) was used to synthesize RNA from DNA templates; and sense and anti-sense 446 Xosr001 RNA transcripts were purified with the MEGAclear Transcription Clean-Up 447 Kit (ThermoFisher, USA). ..

    Article Title: Interactors and effects of overexpressing YlxR/RnpM, a conserved RNA binding protein in cyanobacteria
    Article Snippet: For northern blot verification, extracted RNA was loaded on a 10% polyacrylamide-8.3 M urea gel and electroblotted onto Hybond-N+ nylon membranes (Amersham) with 1 mA per cm 2 for 1 h. RnpB RNA enrichment was analysed by Northern hybridization using single-stranded radioactively labelled RNA probes transcribed in vitro from PCR-generated templates using the respective primers (Supplementary Table S1). .. The radioactively labelled probes were generated using [α- 32 P]-UTP and the Maxiscript T7 In vitro transcription kit (Thermo Fisher Scientific). ..

    Article Title: Suppression of host plant defense by bacterial small RNAs packaged in outer membrane vesicles
    Article Snippet: .. The MAXIscript T7 In Vitro Transcription Kit (Thermo Fisher Scientific) was used to synthesize RNA from DNA templates, and sense and anti-sense Xosr001 RNA transcripts were purified with the MEGAclear Transcription Clean-Up Kit (Thermo Fisher Scientific). ..

    Article Title: The RNA landscape of the human commensal Segatella copri reveals a small RNA essential for gut colonization
    Article Snippet: .. For riboprobe generation, PCR template was obtained for each probe and RNA probes were generated with MAXIscript T7 in vitro transcription kit (Thermo Scientific, AM1312) following manufacturers instruction with minor modifications, and DIG RNA labeling mix (Sigma Aldrich, #11277073910) was added as nucleotide mix. .. Labeled riboprobe were further purified via Microspin G-50 columns (Cytiva, # 27-5330-01).

    Article Title: R-DeeP/TripepSVM identifies the RNA-binding OB-fold-like protein PatR as regulator of heterocyst patterning
    Article Snippet: The RNA was fractionated on a 10% urea-polyacrylamide (PAA) gel, stained with ethidium bromide and electroblotted onto a Hybond-N + membrane (Amersham) at 1 mA per cm 2 for 1 h. The distribution of RnpB, Yfr1 and NsiR8 was analyzed by northern hybridization using single-stranded radioactively labeled RNA probes transcribed in vitro from polymerase chain reaction (PCR)-generated templates (see for primers). .. Radioactively labeled probes were generated using [α- 32 P]-UTP and the Maxiscript T7 In vitro transcription kit (Thermo Fisher Scientific). ..

    Article Title: Interactors and effects of overexpressing YlxR/RnpM, a conserved RNA binding protein in cyanobacteria.
    Article Snippet: For northern blot verification, extracted RNA was loaded on a 10% polyacrylamide-8.3 M urea gel and electroblotted onto Hybond-N+ nylon membranes (Amersham) with 1 mA per cm2 for 1 h. RnpB RNA enrichment was analysed by Northern hybridization using singlestranded radioactively labelled RNA probes transcribed in vitro from PCR-generated templates using the respective primers (Supplementary Table S1). .. The radioactively labelled probes were generated using [α-32P]-UTP and the Maxiscript T7 In vitro transcription kit (Thermo Fisher Scientific). ..

    Purification:

    Article Title: Suppression of host plant defense by bacterial small RNAs packaged in outer membrane vesicles.
    Article Snippet: .. The MAXIscript T7 In Vitro Transcription Kit (ThermoFisher, 445 USA) was used to synthesize RNA from DNA templates; and sense and anti-sense 446 Xosr001 RNA transcripts were purified with the MEGAclear Transcription Clean-Up 447 Kit (ThermoFisher, USA). ..

    Article Title: Suppression of host plant defense by bacterial small RNAs packaged in outer membrane vesicles
    Article Snippet: .. The MAXIscript T7 In Vitro Transcription Kit (Thermo Fisher Scientific) was used to synthesize RNA from DNA templates, and sense and anti-sense Xosr001 RNA transcripts were purified with the MEGAclear Transcription Clean-Up Kit (Thermo Fisher Scientific). ..

    other:

    Article Title: An antisense RNA regulates production of DnaA and affects sporulation in Bacillus subtilis
    Article Snippet: RNA size standards were transcribed using Century Marker Template Plus fragments (Invitrogen by ThermoFisher Scientific, AM7782) and an Ambion MAXIscript T7 In Vitro Transcription Kit (AM1312) according to the manufacturer’s instructions.

    Article Title: R-DeeP/TripepSVM identifies the RNA-binding OB-fold-like protein PatR as regulator of heterocyst patterning.
    Article Snippet: Radioactively labeled probes were generated usng [ α- 32 P]-UTP and the Maxiscript T7 In vitro transcripion kit (Thermo Fisher Scientific).

    Generated:

    Article Title: Interactors and effects of overexpressing YlxR/RnpM, a conserved RNA binding protein in cyanobacteria
    Article Snippet: For northern blot verification, extracted RNA was loaded on a 10% polyacrylamide-8.3 M urea gel and electroblotted onto Hybond-N+ nylon membranes (Amersham) with 1 mA per cm 2 for 1 h. RnpB RNA enrichment was analysed by Northern hybridization using single-stranded radioactively labelled RNA probes transcribed in vitro from PCR-generated templates using the respective primers (Supplementary Table S1). .. The radioactively labelled probes were generated using [α- 32 P]-UTP and the Maxiscript T7 In vitro transcription kit (Thermo Fisher Scientific). ..

    Article Title: The RNA landscape of the human commensal Segatella copri reveals a small RNA essential for gut colonization
    Article Snippet: .. For riboprobe generation, PCR template was obtained for each probe and RNA probes were generated with MAXIscript T7 in vitro transcription kit (Thermo Scientific, AM1312) following manufacturers instruction with minor modifications, and DIG RNA labeling mix (Sigma Aldrich, #11277073910) was added as nucleotide mix. .. Labeled riboprobe were further purified via Microspin G-50 columns (Cytiva, # 27-5330-01).

    Article Title: R-DeeP/TripepSVM identifies the RNA-binding OB-fold-like protein PatR as regulator of heterocyst patterning
    Article Snippet: The RNA was fractionated on a 10% urea-polyacrylamide (PAA) gel, stained with ethidium bromide and electroblotted onto a Hybond-N + membrane (Amersham) at 1 mA per cm 2 for 1 h. The distribution of RnpB, Yfr1 and NsiR8 was analyzed by northern hybridization using single-stranded radioactively labeled RNA probes transcribed in vitro from polymerase chain reaction (PCR)-generated templates (see for primers). .. Radioactively labeled probes were generated using [α- 32 P]-UTP and the Maxiscript T7 In vitro transcription kit (Thermo Fisher Scientific). ..

    Article Title: Interactors and effects of overexpressing YlxR/RnpM, a conserved RNA binding protein in cyanobacteria.
    Article Snippet: For northern blot verification, extracted RNA was loaded on a 10% polyacrylamide-8.3 M urea gel and electroblotted onto Hybond-N+ nylon membranes (Amersham) with 1 mA per cm2 for 1 h. RnpB RNA enrichment was analysed by Northern hybridization using singlestranded radioactively labelled RNA probes transcribed in vitro from PCR-generated templates using the respective primers (Supplementary Table S1). .. The radioactively labelled probes were generated using [α-32P]-UTP and the Maxiscript T7 In vitro transcription kit (Thermo Fisher Scientific). ..

    Polymerase Chain Reaction:

    Article Title: The RNA landscape of the human commensal Segatella copri reveals a small RNA essential for gut colonization
    Article Snippet: .. For riboprobe generation, PCR template was obtained for each probe and RNA probes were generated with MAXIscript T7 in vitro transcription kit (Thermo Scientific, AM1312) following manufacturers instruction with minor modifications, and DIG RNA labeling mix (Sigma Aldrich, #11277073910) was added as nucleotide mix. .. Labeled riboprobe were further purified via Microspin G-50 columns (Cytiva, # 27-5330-01).

    Labeling:

    Article Title: The RNA landscape of the human commensal Segatella copri reveals a small RNA essential for gut colonization
    Article Snippet: .. For riboprobe generation, PCR template was obtained for each probe and RNA probes were generated with MAXIscript T7 in vitro transcription kit (Thermo Scientific, AM1312) following manufacturers instruction with minor modifications, and DIG RNA labeling mix (Sigma Aldrich, #11277073910) was added as nucleotide mix. .. Labeled riboprobe were further purified via Microspin G-50 columns (Cytiva, # 27-5330-01).

    Article Title: R-DeeP/TripepSVM identifies the RNA-binding OB-fold-like protein PatR as regulator of heterocyst patterning
    Article Snippet: The RNA was fractionated on a 10% urea-polyacrylamide (PAA) gel, stained with ethidium bromide and electroblotted onto a Hybond-N + membrane (Amersham) at 1 mA per cm 2 for 1 h. The distribution of RnpB, Yfr1 and NsiR8 was analyzed by northern hybridization using single-stranded radioactively labeled RNA probes transcribed in vitro from polymerase chain reaction (PCR)-generated templates (see for primers). .. Radioactively labeled probes were generated using [α- 32 P]-UTP and the Maxiscript T7 In vitro transcription kit (Thermo Fisher Scientific). ..



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